Complete genome sequences of novel papillomaviruses in captive black-... - SRA

SRX17293739: Complete genome sequences of novel papillomaviruses in captive black-and-white ruffed lemurs (Varecia variegata )
1 ILLUMINA (Illumina NovaSeq 6000) run: 6,785 spots, 2M bases, 713,680b downloads

Design: Saliva samples were collected by allowing the lemurs to chew on a SalivaBio Childrens Swab (Salivametrics, USA). The saturated swabs were then placed into a SalivaBio Swab Storage Tube (Salivametrics, USA) and centrifuged to collect the saliva at the bottom of the storage tube. Saliva samples were stored at -80 until viral DNA extraction. To the saliva sample, SM buffer (0.1M NaCl, 50mM Tris-HCl [pH 7.4]) was added in order to obtain 400 l of solution per sample. 200 l of solution per sample was run through the High Pure Viral Nucleic Acid Kit (Roche Diagnostics, USA) to perform viral DNA extraction. 1l of the extracted viral DNA was amplified using the Illustra TempliPhi rolling circle amplification kit (GE Healthcare, USA) to target circular viruses. The viral DNA and rolling amplified viral DNA were combined. Libraries were generated using the Illumina DNA Prep kit which utilizes tagmentation to fragment the DNA. Reactions were performed at a reduced 1/8 volume reaction on the SPT Labtech HV Mosquito and Dragonfly Discovery liquid handlers. Libraries were sequenced using the Illumina NovaSeq 6000 on an SP flowcell (2x150bp).

Submitted by: Arizona State University

Study: Lemur viral dynamics project

PRJNA874427 • SRP394565 • All experiments • All runs

show Abstracthide Abstract

Identification of viruses associated with lemurs both in the wild and captivity

Sample:

SAMN30547858 • SRS14858052 • All experiments • All runs

Organism: viral metagenome

Library:

Name: D23SF_viral

Instrument: Illumina NovaSeq 6000

Strategy: WGA